STIM1 抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
STIM1 抗体

STIM1 抗体

商家询价

产品名称： STIM1 抗体

英文名称： STIM1 Antibody

产品编号： XY11565-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 STIM1 抗体Applications

In-house applications ELISA, WB, IP, IHC, IF, FC     Application not tested? Please see our Guarantee.
Published applications IF, IHC, IP, WB Publications 12 citations –view here
Species specificity Human, Mouse; other species not tested.
Published Species Human, mouse, rat
Images
Prev12NextView more validation data
  • 11565-1-AP;Jurkat cellsJurkat cells were subjected to SDS PAGE followed by western blot with 11565-1-AP(STIM1 antibody) at dilution of 1:300
  • 11565-1-AP;Raji cellsIP Result of anti-STIM1 (IP:11565-1-AP, 3ug; Detection:11565-1-AP 1:1000) with Raji cells lysate 3800ug.
  • 11565-1-AP;human ovary tumor tissueImmunohistochemical of paraffin-embedded human ovary tumor using 11565-1-AP(STIM1 antibody) at dilution of 1:50 (under 40x lens)
  • 11565-1-AP;HepG2 cellsImmunofluorescent analysis of HepG2 cells using 11565-1-AP( STIM1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
  • 11565-1-AP;HepG2 cells1X10^6 HepG2 cells were stained with .2ug STIM1 antibody (11565-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in Jurkat cells, BxPC-3 cells, HT-1080 cells, mouse liver tissue, NIH/3T3 cells, Raji cells
Positive IP detected in Raji cells
Positive IHC detected in Human ovary tumor tissue, human lung cancer tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:200-1:2000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100
 STIM1 抗体AApplication key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information STIM1 抗体A
Immunogen STIM1 fusion protein ag2140 Full name Stromal interaction molecule 1
Calculated molecular
weight		 685aa, 77 kDa Observed molecular 
weight		 80-90 kDa
GenBank accession 
number		 BC021300 Gene ID (NCBI) 6786
Gene symbol STIM1 Synonyms D11S4896E, GOK, STIM1, stromal interaction molecule 1
Background

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER–plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1. STIM1 抗体A

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