PLK4 (Human) Recombinant Protein
产品名称： PLK4 (Human) Recombinant Protein
英文名称： PLK4 (Human) Recombinant Protein
产品编号： P5799
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Human PLK4 (BAG36907.1, 1 a.a. - 970 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 136
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 70 % by SDS-PAGE/CBB staining
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG.Substrate : MBP. ATP: 100 µM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- SDS-PAGE Stained with Coomassie Blue
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 10733
- Protein Accession#:
- BAG36907.1
- Gene Name:
- PLK4
- Gene Alias:
- SAK,STK18
- Gene Description:
- polo-like kinase 4 (Drosophila)
- Omim ID:
- 605031
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. [provided by RefSeq
- Other Designations:
- Snk akin kinase,polo-like kinase 4,serine/threonine kinase 18,serine/threonine protein kinase SAK
- Related Disease